vibrio alginolyticus pronunciation

Attenuated strain V. alginolyticusvscC was selected on TCBS+Cm plates followed by a 10% sucrose selection process. 2010;70(1): 205-209. US English. Donaghy L, Hong H-K, Lee H-J, Jun J-C, Park Y-J, Choi K-S. Hemocyte Parameters of the Pacific Oyster Crassostrea Gigas a Year After the Hebei Spirit Oil Spill Off the West Coast of Korea. TBtools: An Integrative Toolkit Developed for Interactive Analyses of Big Biological Data. The plates were processed to remove residual liquid in the wells after wash step. doi:10.1016/j.redox.2020.101508, 45. First published: 31 October 2011 https://doi.org/10.1111/j.1365-2109.2011.03013.x Citations: 41 Read the full text PDF Tools Share Abstract Asian seabass ( Lates calcarifer) has been designated as a candidate species for open sea cage culture in India. doi:10.1016/s0044-8486(03)00215-1, 18. Lake Superior State University, United States, Institute of Oceanology, Chinese Academy of Sciences (CAS), China. Statistical analysis was performed with FlowJo (v10.0 software). Hernandez-Robles MF, Alvarez-Contreras AK, Juarez-Garcia P, Natividad-Bonifacio I, Curiel-Quesada E, Vazquez-Salinas C, et al. WebGI, wound infections, septicemia. In mammalian cells, since activation of TLRs serves to promote innate responses and initiate adaptive immunity (82, 83), such features are of interest because these pathways can be exploited as potential vaccine adjuvants (84, 85). WebConclusions: Vibrio alginolyticus harbours functional luxO gene that regulates the secretion of extracellular protease and haemolytic materials as well as siderophore production in either sigma(54) dependent or independent manners. Vibriosis (Vibrio other, Vibrio alginolyticus, Vibrio cholerae type non-O1, Vibrio cholerae type non-O139, non-toxigenic Vibrio cholerae type O1 or O139, Vibrio fluvialis,Grimontia hollisae, Vibrio mimicus, Vibrio parahaemolyticus, Vibrio vulnificus) Note: This guidance is intended for management of non-toxigenic strains of V. cholerae O1 and We believe that attenuated pathogen strains such as that of virulent Vibrio species in applications deserve more attention, as immune priming in the oyster has been launched during the last few years for exploration of immunity system evolution to prevent disease outbreaks (8993). Una prdida del 10 % o ms del peso corporal indica una deshidratacin grave. doi:10.1007/s10152-010-0190-7, 24. Hemocytes were harvested after bacterial exposure to oysters in vivo. For cGMP assay, oyster hemocytes were collected in 1.5 ml centrifuge tubes after in vivo infection, following by centrifugation and resuspension in PBS. During the acclimation period, oysters were fed with the microalgae Isochrysis galbana (105 cells/ml) and Chaetoceros calcitrans (2 105 cells/ml) twice a day. and its immunomodulation effects on apoptosis in the oyster. Its ability to survive in such extreme environments is dependent on an open yet sophisticated circulation system that executes innate immune functions and allows unique strategies for stress adaptation, making oysters suitable biomarkers for health assessment of marine ecosystems (20, 21). US English. ZY, YZ, and FM designed this study. We generated a Vibrio alginolyticus mutant strain (V. alginolyticusvscC) with attenuated virulence by knocking out the vscC encoding gene, a core component of type III secretion system (T3SS), which led to starkly reduced apoptotic rates in hemocyte hosts compared to the V. alginolyticusWT control. Front Cell Infect Microbiol (2020) 10:183. doi:10.3389/fcimb.2020.00183, 16. Manage Settings Upon treatment with H2O2 at varying concentrations, viability of hemocytes was determined by the MTT assay, which is based on absorbance measurement at 570 nm for the conversion of MTT into formazan crystals by living cells. Price M, Terlecky SR, Kessel D. A Role for Hydrogen Peroxide in the Pro-Apoptotic Effects of Photodynamic Therapy. WebHow to pronounce vibrio alginolyticus? Q1: necrotic/non-viable cells; Q2: late apoptosis/secondary necrosis; Q3: early apoptosis; Q4: living cells. Within its native habitat, C. hongkongensis is constantly exposed to microbial assaults in a dynamic intertidal environment (18, 19). doi:10.1016/j.molp.2020.06.009, 28. 19458. Vibrio alginolyticus is a halophilic organism formerly regarded as biotype 2 of V. parahaemolyticus. Each oyster was injected with 50 g dsRNA. Three days after injection, five individuals from each group were picked randomly for collection of hemocytes to obtain biological replicates. The GO enrichment was performed by using annotation of differentially expressed proteins (DEPs) with a hypergeometric test. 12. doi:10.1038/nrc2723, 53. Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2(-Delta Delta C(T)) Method. All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Expression level of sGC mRNA was also detected in hemocytes after Vibrio infection, and results showed that only V. alginolyticusvscC strain but not V. alginolyticusWT strain infection could lead to significant downregulation of sGC (Supplementary Figure5). doi:10.1016/j.dci.2014.02.005, 69. Int J Sci Technol. https://www.definitions.net/pronounce/vibrio+alginolyticus. We're doing our best to make sure our content is useful, accurate and safe.If by any chance you spot an inappropriate comment while navigating through our website please use this form to let us know, and we'll take care of it shortly. The numbers of specific and common DEPs for injection of the two Vibrio strains are as indicated. Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae; Vibrio, Organisms from the Vibrio genus were first observed by Filippo Pacini in 1854, and were further described by Robert Koch in 1883. [1][2] It is medically important since it causes otitis and wound infection. Three biological replications were performed in each assay, except for five replications for RNA interference assay. doi:10.1099/vir.0.070011-0, 76. Fred. Complete Genome Sequence of Vibrio alginolyticus ZJ-T. Genome Announcements. Intuitively, it would be interesting to compare this to host response during challenge of an attenuated Vibrio strain. Subsequently, exogenously applied cGMP was injected into the oysters via the adductor muscle to test whether excess cGMP levels would cause oyster hemocyte apoptosis. Remarkably, our analysis indicates that challenge with the virulent strain triggered off production of intracellular O2, which was involved in early apoptosis (42). Mukherjee S, Huda S, Sinha Babu SP. The main parameters are set as follows: electrospray voltage, 1.6kV; the first-order MS/MS scan, from 350 to 1,600 m/z at resolution of 70,000; the second-order MS/MS scan, 100 m/z at resolution of 17,500. It was noted that the mortality of the fish increased with increased concentration of V. alginolyticus, where infected fish were able to infect healthy fish. Infect Immun (2002) 70(2):9649. The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. sGC is the primary sensor of nitric oxide (NO), binding of which boosts the enzymatic activity (6064). NO-Independent Stimulators and Activators of Soluble Guanylate Cyclase: Discovery and Therapeutic Potential. Nat Rev Cancer (2009) 9(10):74958. Overall, our findings demonstrate that infection by virulent Vibrio strains activate respiratory bursts-dependent apoptosis in oyster hemocytes, with the induction and activation of p53 signaling pathways. Fitness Costs Associated With Maternal Immune Priming in the Oyster. The consent submitted will only be used for data processing originating from this website. 8. 1973 ). Data were analyzed by one-way ANOVA and presented as mean SEM (n=3); *p < 0.05; **p < 0.01. J Gen Virol (2014) 95(Pt 12):271626. Kitamura Y, Ota T, Matsuoka Y, Nomura Y, Gebicke-haerter PJ, Taniguchi T. Hydrogen PeroxideInduced Apoptosis Mediated by P53 Protein in Glial Cells. Zhou L, Yang F, Li G, Huang J, Liu Y, Zhang Q, et al. Zhang G, Li L, Meng J, Qi H, Qu T, Xu F, et al. Each assay was performed in triplicates with GADPH mRNA as an internal control. Chen C, Chen H, Zhang Y, Thomas HR, Frank MH, He Y, et al. Aproximadamente una docena de especies de Vibrio pueden causar enfermedad en los seres humanos, la cual se conoce como vibriosis. These results suggest that the virulent Vibrio strain induced intracellular cGMP generation, which modulated the outcomes of hemocytes apoptosis. Furchgott RF, Jothianahdan D. Endothelium-Dependent and -Independent Vasodilation Involving Cyclic GMP: Relaxation Induced by Nitric Oxide, Carbon Monoxide and Light. Cold Spring Harb Protoc (2018) 2018(6):14. FM, KL, and N-KW performed the data analyses and drafted the manuscript. Harrison's Principles of Internal Medicine 18th edition. doi:10.1038/s41392-020-0196-9, 56. Definitions.net. You have earned {{app.voicePoint}} points. doi: 10.1016/S0005-2728(99)00024-9, 72. Trends Microbiol (2016) 24(7):56880. This Gammaproteobacteria-related article is a stub. Then, an RNA interference assay was performed for in vivo knockdown of the expression of Chp53 target protein to clarify the functional meaning of Chp53 target protein as a signaling target during induction of apoptosis in oyster hemocytes. 1961, Vibrio alginolyticus is a Gram-negative marine bacterium. In recent years, research on the pathogenic mechanisms of V. alginolyticus has gained momentum, which enables the identification of some key virulence factors including polar and lateral flagella, hemolysins, alternative sigma factor RpoX, outer membrane protein A (OmpA), type III secretion system, and so on (3, 10, 11). Being an ecologically relevant host, the oyster Crassostrea hongkongensis can serve as an excellent model for elucidating mechanisms underlying host-Vibrio interactions. Specifically, V. alginolyticusWT challenge induced elevated expression of 10 proteins and blunted the expression of three proteins, namely, superoxide dismutase (SOD), metallothionein IV, and cell death-inducing p53-target protein 1, which are involved in respiratory bursts and apoptosis during infection (Figure3C) (31). Golenishcheva EN. Lennicke C, Rahn J, Lichtenfels R, Wessjohann LA, Seliger B. Hydrogen Peroxide - Production, Fate and Role in Redox Signaling of Tumor Cells. Among these, a total of 17 significant GO terms were found to be statistically enriched, which are involved in processes of host immune defenses including cell apoptosis, energy metabolism, cytoskeleton remodeling, and phagocytosis processing (Figure3A). https://www.definitions.net/pronounce/vibrio alginolyticus. Notably, mitochondrial O2 levels in the V. alginolyticusWT injection group rose significantly 3 h following challenge by the V. alginolyticusWT strain, culminated at 12 h post-challenge. Martins ML, Mourio JLP, Fezer GF, Buglione Neto CC, Garcia P, Silva BC, Jatob A, Vieira FN. Through a proteomic approach, we determined differentially expressed proteins (DEPs) in response to infection by different virulent Vibrio strains, and analyzed the trends of how such DEPs regulate immune outcomes in oysters. 1. Vibrio species are ubiquitously distributed in marine environments, with important implications for emerging infectious diseases. How Diversity may Compensate for Scarcity in the Regulation of Resident/Pathogenic Microflora. Walters ET, Lewin MR. Cyclic GMP Pathway is Critical for Inducing Long-Term Sensitization of Nociceptive Sensory Neurons. Forty oysters were randomly assigned into four groups and placed into four tanks: dsGFP + PBS, dsGFP + V. alginolyticusWT, dsp53tp + PBS, dsp53tp + V. alginolyticusWT. The late apoptotic cells were in the upper right area co-stained with FITC and PI (FITC+/PI+, Q2). Seems like your pronunciation of vibrio alginolyticus is not correct. Protein annotations were predicted by using the Blast2GO tool in the Gene Ontology (GO) platform (http://www.geneontology.org/). of different pathogenicity, we examined protein expression patterns following challenges with V. alginolyticusWT or V. alginolyticusvscC. Vibrio alginolyticus is a Gram-negative bacterium ubiquitously found in aquatic and marine habitats and poses considerable health risks to marine animals and humans alike via the local food-chains (1). The history of seafood exposure is a major feature of V. alginolyticus infection. Bello OD, Jouannot O, Chaudhuri A, Stroeva E, Coleman J, Volynski KE, et al. Lacroix M, Riscal R, Arena G, Linares LK, Le Cam L. Metabolic Functions of the Tumor Suppressor P53: Implications in Normal Physiology, Metabolic Disorders, and Cancer. Protein cluster of V. alginolyticusvscC-specific DEPs consists of 16 proteins, of which 10 were specifically upregulated and 6 downregulated. sGC-cGMP is a pro-apoptotic pathway induced, and cGMP can promote apoptosis (38). To examine more closely the mechanisms underlying V. alginolyticusWT-induced hemocytes apoptosis, we assessed the levels of intracellular cGMP, after infection with the V. alginolyticusWT strain or V. alginolyticusvscC strain.

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